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We narrowed to 180 results for: antibody

Showing: 101 - 120 of 180 results

  1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...beads. Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A...Immunoglobulin G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since ...is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior to the ...

  2. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids...below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them...

  4. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While...

  5. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody that...that targets your protein of interest or primary antibody respectively. Avidin bound to a reporter is then... on this complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...protein visualized with a fluorescently-tagged antibody. By employing “marker proteins” which are known... of a cell. This mapping can be done by using antibodies targeted to the marker proteins, however, in ... cell membrane permeabilized in order for the antibodies to reach their intracellular targets. Therefore...

  9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer Lab published ...distributing 94 plasmids expressing validated antibodies for neuroscience research (see the NeuroMab Collection...specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved ...

  11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...that SpyLigase can be used to link affibodies or antibodies against common tumor markers to subsequently ...approaches to improve CTC capture, but I hope that our antibody tentacles may have an impact on this challenge...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell...

  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through...ATLAS (anterograde transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold... Arnold Lab, ATLAS is based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...advantages over their amino acid-based counterparts, antibodies and enzymes, such as: Design and selection ...commercial synthesis (those of us who have used antibodies for diagnostics know how frustrating it can be...hybridization (FISH) where fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise...

  14. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...transcription factor IRF-1. Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers...well as affinity purification with an anti-Cas9 antibody. These different tag systems and affinity purification...similar affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby excitation...required. These dyes can also be attached to an antibody to detect various targets, such as proteins, nucleic...568- and Fluorescein Isothiocyanate- conjugated Antibody. Cell Journal, 13(3), 169–172. Salari, M., Bitounis...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration...for AAV due to the development of neutralizing antibodies in animal models. All of our engineered AAV are...

  18. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...who have complaints about the unreliability of antibodies. IPI is working to address this issue directly...directly by creating well characterised antibodies against all human extracellular proteins with community-...research tools. BenchSci collates information about antibodies while FPbase curates information about fluorescent...

  20. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...
Showing: 101 - 120 of 180 results