We narrowed to 236 results for: cloning

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

Sept. 14, 2021, 1:15 p.m.

Scientists from UCSF identify 332 human proteins that the SARS-CoV-2 virus interacts with in cells in attempts to find druggable targets.

April 9, 2020, 1:15 p.m.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Aug. 6, 2024, 1:15 p.m.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

March 3, 2020, 2:15 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

March 23, 2021, 1:15 p.m.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Sept. 10, 2024, 1:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.

June 17, 2021, 1:17 p.m.

The Addgene blog has expanded tremendously in 2015. Check out our curated list of our top viewed posts from 2015!

Dec. 7, 2015, 6 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

Feb. 8, 2024, 2:15 p.m.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

June 13, 2016, 7:08 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Welcome to Addgene's blog! In our blog we'll help you share science by highlighting the latest plasmid technologies, bringing you the inside scoop from scientists, providing career development advice, and more.

Nov. 14, 2013, 4:10 p.m.

A review of the advantages of epitope tags and commonly used tags and their antibodies.

May 11, 2023, 1:15 p.m.