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We narrowed to 228 results for: cloning

Showing: 101 - 120 of 228 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...were used in this study.  The zCas9i cloning kit is a plant cloning kit that contains a series of vectors...CRISPR-based lineage tracing tool captures rare clones that represent 0.001% of the population.  Because...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...terminus the tag will end up on after successful cloning. Cloning information: This section provides useful ...plasmid's structural features and see if I can easily clone my gene into it? Your best bet is the Plasmid ...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on...Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...complex eukaryotic cells, but would prefer to grow, clone, and manipulate their vectors in fast-growing bacteria...example, if you are using E. coli simply for easy cloning and quick DNA production, you probably don’t need...called transfer vectors. The gene of interest is cloned into a shuttle vector for transfection into producer...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ... available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids have... be produced in insect cells, but they require cloning specific AAV or lentiviral machinery into a baculovirus...bacmids are readily available, so the amount of cloning required is about the same between insect and mammalian...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning Read..., as detected by qPCR, but this did not reduce cloning efficiencies.  When compared to fresh columns, ...

  7. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...

  9. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...in your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

  12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...samples. For a more detailed comparison of these cloning strains, browse the table of common gene mutations...N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...knock-in at a genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you...developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene...

  14. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of...transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation of naturally... they are just spontaneous antibiotic resistant clones. But if they are blue or magenta, they are real...

  15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...protein. These were either (i) coded by the multiple cloning site and therefore consist of random amino acid... often use restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent...PVAT, when we use the AgeI site in the multiple cloning site) is sufficient. For the fluorescent protein-POI...

  16. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...monoclonal antibodies are derived from a single B cell clone. One common way of producing monoclonal antibodies...freeze hybridoma cells before culturing or they can clone the genes from the hybridoma into a plasmid to create...

  18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin selection...either side of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more recombinogenic ...inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the gRNA cassette, one ...and the gRNA are Tet-inducible, and a gRNA can be cloned easily into pRH2521 using BbsI. This system results...syringae. These vectors contain BsaI sites for easy cloning of a gRNA of interest. Cytidine and adenine base...
Showing: 101 - 120 of 228 results