We narrowed to 128 results for: pcr

Learn about the differences in virus production between insect cells and mammalian cells.

Nov. 5, 2024, 8:32 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Sept. 9, 2014, 8:20 p.m.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

July 29, 2025, 1:15 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

Jan. 26, 2023, 2:15 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

A laboratory research management consultant and coach provides step-by-step advice on how to negotiate a budget when stating your lab.

Sept. 1, 2015, 2:30 p.m.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

May 13, 2025, 1:15 p.m.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

May 20, 2021, 1:15 p.m.

Learn about CRISPR plant base editors for plants

Jan. 3, 2019, 1:35 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

June 23, 2016, 2:30 p.m.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

May 10, 2016, 2:30 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.