We narrowed to 296 results for: promoter

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

Learn all about our top requested plasmid from 2017 - lentivirus envelope vector pMD2.G encoding VSG-G.

Jan. 10, 2018, 4:13 p.m.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

April 7, 2015, 4:21 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

Chromoproteins are a subset of the fluorescent protein family. They give strong color in visible light and can be used for molecular biology experiments.

Feb. 4, 2020, 2:15 p.m.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

Feb. 25, 2016, 3:30 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

Dec. 16, 2014, 8:01 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. Read more to learn about the recipients of the Addgene-Seeding Labs plasmid grant this year.

Nov. 27, 2018, 2 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. In this post, we share 20 new viral preps we've added in the last quarter.

July 9, 2024, 1:15 p.m.

We are excited to officially re-launch our redesigned AAV Data Hub, with easy submission and DOIs to help share science!

Feb. 7, 2023, 2:15 p.m.

Tips for naming your plasmid in a way that is clear and informative to you and your colleagues.

Feb. 13, 2014, 1 p.m.

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

Jan. 24, 2023, 2:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

Joanne Kamens and Tyler Ford discuss the benefits of using Reddit to communicate your science.

Oct. 20, 2016, 2:30 p.m.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Aug. 18, 2016, 2:30 p.m.

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

April 5, 2022, 1:20 p.m.