We narrowed to 173 results for: GFP

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

Dec. 13, 2018, 1:14 p.m.

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

Feb. 14, 2018, 2:36 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Overview of the MORF library collection and accompanying ATLAS deposited at Addgene

March 30, 2023, 1:15 p.m.

Scientists from UCSF identify 332 human proteins that the SARS-CoV-2 virus interacts with in cells in attempts to find druggable targets.

April 9, 2020, 1:15 p.m.

Learn about new and upcoming viral vectors from Addgene including our new trial size AAVs for serotype testing, calcium and GABA sensors, and more!

Sept. 12, 2019, 1 p.m.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

June 4, 2019, 1:02 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

March 25, 2014, 3:15 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

See the winners for the 2021 Michael Davidson and Roger Tsien Commemorative Conference Award.

Aug. 5, 2021, 12:15 p.m.

Learn how you can use photoswitchable kinases to control signaling pathways with light!

July 25, 2017, 1:20 p.m.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Aug. 10, 2023, 1 p.m.

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

June 22, 2023, 1:15 p.m.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Dec. 14, 2021, 2:15 p.m.

Learn how you can use split-BioID to study protein-protein interactions and build a better understand of your protein's interactome.

May 1, 2018, 1:57 p.m.