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We narrowed to 181 results for: antibody

Showing: 121 - 140 of 181 results

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby excitation...required. These dyes can also be attached to an antibody to detect various targets, such as proteins, nucleic...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...activity (Ni, Titov, & Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013),...synthesized with reagents used to fluorescently label antibodies, and purification is a snap using thin layer ... et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS ...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...with the most alternative functions — there are antibodies to it and ways to achieve fluorescence with it...Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

  6. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...additional data regarding the specificity of this antibody?” You will also help troubleshoot products, give... in a Western Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... The benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...

  8. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells...types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia are...

  9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens...Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging...

  10. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Multimerization in the SunTag system occurs via antibody-peptide labeling; specifically, cognate peptide...recognized and thus fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies the intensity...scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional activation domains...more - all without the need to develop custom antibodies. Unfortunately, while there are many types of...proteins, ordered structures, and accessibility to an antibody. After identifying possible protein domains containing...containing these properties, they generated monoclonal antibodies against them, fused them to GFP, and compared...

  15. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...native protein context, but they still depend on antibody-based detection or western blotting, methods that... degradation, trafficking (Hall et al., 2012). Antibody-based tools can also be combined with the HiBiT...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...characterization, these proteins could function as surrogate antibodies for identifying and characterizing sugars and...temperature. If you’re lucky enough to have an antibody or other protein on hand that will specifically...

  17. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. This...

  20. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... or purify it – and you do not have a specific antibody against the protein available – you can always...
Showing: 121 - 140 of 181 results