We narrowed to 236 results for: cloning

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

May 20, 2025, 1:15 p.m.

Chromoproteins are a subset of the fluorescent protein family. They give strong color in visible light and can be used for molecular biology experiments.

Feb. 4, 2020, 2:15 p.m.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

Dec. 16, 2014, 8:01 p.m.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Nov. 12, 2014, 3:54 p.m.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

April 16, 2024, 1:15 p.m.

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

June 15, 2016, 1:30 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids.

July 22, 2025, 1:15 p.m.

In the final post of our Quick Guide to Working with Drosophila, learn how to make Drosophila transgenic lines.

July 28, 2017, 1:30 p.m.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Sept. 22, 2015, 2:30 p.m.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Jan. 28, 2014, 3:10 p.m.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

July 14, 2021, 1:15 p.m.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Sept. 29, 2020, 1:15 p.m.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Aug. 15, 2019, 12:30 p.m.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

June 17, 2014, 7:57 p.m.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Jan. 8, 2014, 3:10 p.m.

Learn about new tools in Addgene's collection, including new prime editing and CRISPR applications, tools for neuroscience research, and more!

Sept. 1, 2026, 1:15 p.m.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

April 6, 2023, 3 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.