We narrowed to 701 results for: plasmid

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Feb. 16, 2023, 7:05 p.m.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

March 14, 2024, 1:15 p.m.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

May 6, 2014, 1:07 p.m.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

June 25, 2019, 12:54 p.m.

Learn how repressible promoters work and how you can use them in your next experiment.

Dec. 20, 2022, 2:15 p.m.

Learn about the advantages of secondary nanobodies from the Gorlich lab over traditional antibodies. Applications include one-step fluorescent immunostaining.

Feb. 27, 2018, 2:04 p.m.

In the second episode of our Hot plasmids podcast, learn about new RFPs, AAV tools, and plasmids for generating transgenic zebrafish.

Sept. 8, 2017, 7:52 p.m.

Light-inducible transcriptional effectors (LITEs) have been designed by Feng Zhang's lab to bind specific genes and turn them on or off in response to light.

Nov. 21, 2013, 3:56 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

May 2, 2023, 1 p.m.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Oct. 27, 2016, 2:30 p.m.

An introduction to Gram negative and positive bacteria - what are they, how can you tell the difference, and where did this terminology come from?

Aug. 2, 2022, 1:15 p.m.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

March 12, 2024, 1:15 p.m.

Changes in export control regulations have make rabies and VSV-G plasmids more accessible to researchers around the world.

Feb. 6, 2025, 2:15 p.m.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

May 22, 2018, 1:38 p.m.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Dec. 3, 2019, 11:38 a.m.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

April 5, 2016, 2:30 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.