Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 228 results for: cloning

Showing: 141 - 160 of 228 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...NNN) at any given codon site of interest can be cloned into an expression vector with Gibson assembly....Both produced about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for...

  2. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually... (DE3)** Rifampicin RecA-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12...

  4. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...around 20 minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome ...out the GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in ...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...developed for another decade when in 1988 they were cloned by two separate labs (Bird et al., 1988; Huston...an scFv?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... al., 2017). Our vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a...single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target...

  10. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...followed by BbsI restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...

  15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...researchers to insert targeting oligos with minimal cloning required. As with other model systems, multiple...containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified...

  16. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...

  18. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...Options”, including adding adapter sequences for cloning purposes.  Press run and wait for the generation...this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences...

  20. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...
Showing: 141 - 160 of 228 results