We narrowed to 236 results for: cloning

Three useful tips to help you organize your lab notebooks, keep track of tasks, and share protocols with other lab members.

Jan. 2, 2014, 3:44 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Read updates on new developments in cancer research and learn about Addgene's Cancer Collection pages.

Sept. 6, 2016, 2:30 p.m.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

May 29, 2014, 1:29 p.m.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Nov. 19, 2013, 2:37 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

Nov. 19, 2025, 2:15 p.m.

2024 is the Year of Camelids! To celebrate, we are taking a look at how these species (e.g. camels, llamas, alpacas) have impacted biological research.

May 9, 2024, 1:15 p.m.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

May 21, 2020, 1:15 p.m.

Learn how to select the plasmid purification kit that best suits your needs!

May 27, 2025, 1:15 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

Sept. 17, 2020, 1:15 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Find details on five methods to quantify DNA: UV absorbance, fluorescence dyes, agarose gel electrophoresis, capillary electrophoresis, and diphenylamine.

July 21, 2020, 1:15 p.m.

Learn how you can use the Build Your Plasmid card game to teach undergraduate students about genetic engineering.

July 17, 2018, 12:23 p.m.

Learn about SnapGene's feature database and how it can be used to identify sequence elements in your plasmids.

Dec. 21, 2017, 2:06 p.m.

Read advice for grad students and postdocs applying for science careers in sales and marketing, including tailoring your CV.

Aug. 21, 2014, 3:15 p.m.

A review of K. phaffii as a model organism and its relevant applications for biotech to basic research.

April 4, 2023, 1 p.m.

Find out what CRISPR tools are distributed as a part of Addgene's new viral service.

Dec. 6, 2016, 3:30 p.m.