We narrowed to 296 results for: promoter

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Aug. 18, 2020, 1:15 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

May 19, 2016, 2:30 p.m.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Dec. 2, 2014, 7:23 p.m.

RUBY-red is a new plant marker that uses a bright red color for easy identification of transgenic plants.

March 1, 2022, 2:15 p.m.

Addgene's AAV Data Hub is a place for researchers to find and share experimental parameters of using AAV. such as titer, injection site, and more.

March 5, 2020, 2:15 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including optogenetics AAV, control AAV, biosensor AAV, and more.

Dec. 19, 2019, 2:40 p.m.

Learn all about a highly processive reverse transcriptase, a new nanobody toolkit, and CRISPR-based tools for monitoring protein-DNA interactions

June 27, 2018, 1:16 p.m.

SunTag amplifies fluorescent signals by recruiting multiple copies of GFP. SunTag can help you turn up the brightness in your fluorescent imaging experiments.

March 28, 2017, 2:30 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

April 11, 2024, 1:15 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Jan. 29, 2019, 2:27 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Sept. 21, 2021, 1:15 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

Addgene Executive Director provides advice on making the decision between academia and non-academia.

March 9, 2017, 3:40 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Learn about items from Addgene's viral service including lentiviral preps expressing SARS-CoV-2 proteins, ChR2 for targeted expression, DREADDs, GFP dependent Cre and FLPo, and more.

May 28, 2020, 1:15 p.m.