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Addgene

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We narrowed to 288 results for: promoter

Showing: 141 - 160 of 288 results

  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...DNA encoding plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this...

  2. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be more cumbersome... vectors integrate into the genome, they could promote oncogenesis by altering local gene expression. ...

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...contains a reporter gene cloned downstream of a promoter containing the target sequence of the DBD. The...as the “prey”) in close enough proximity to the promoter of the reporter gene to activate expression (Figure...

  4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...hesitation. I hope I will be able to effectively promote Addgene, which is also beneficial to my research...Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using...

  5. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) ...

  6. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids...

  8. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...(which you can filter by serotype, species, or promoter) can save you significant time and money. For ...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...transcription by placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores ... RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely transcribed by RNA Polymerase...

  13. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... pages, we added curation fields for purpose, promoters, expression types, and more, all of which help...How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid ...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...resistance cassettes (Prior et al. 2010), inducible promoters with different degrees of stringency and inducibility...Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli ...

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Cas9 would have an off-target that lands in the promoter of another gene, thereby driving aberrant transcription... said, we generally pick guides by putting the promoter of the gene into a gRNA finder such as WU-CRISPR...

  18. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to add a ... start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start site ...of the crRNA) is also expressed from a separate promoter. In the pre-processing form, the crRNA contains...

  19. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper in PLoS One...
Showing: 141 - 160 of 288 results