We narrowed to 294 results for: promoter

... VchINT, three separate plasmids and multiple promoters were used to express the necessary components ...required genes and the CRISPR RNA under the same promoter, and a donor plasmid (pDonor) encodes the mini-transposon...

...hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots. Find the...

...a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be more cumbersome... vectors integrate into the genome, they could promote oncogenesis by altering local gene expression. ...

...contains a reporter gene cloned downstream of a promoter containing the target sequence of the DBD. The...as the “prey”) in close enough proximity to the promoter of the reporter gene to activate expression (Figure...

...hesitation. I hope I will be able to effectively promote Addgene, which is also beneficial to my research...Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using...

...types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) ...

...Competent Cells By Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids...

...a) the bacterial antibiotic resistance, b) the promoter that drives the insert, and c) any other selection...

...(which you can filter by serotype, species, or promoter) can save you significant time and money. For ...

... pages, we added curation fields for purpose, promoters, expression types, and more, all of which help...How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid ...

... vector is inserted downstream of a functional promoter (gene traps). The resultant gene targeting system...pAAV-TK-Acceptor has a conventional heterologous promoter-driven TK-neoR gene. To modify these tagging vectors...pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances...

...transcription by placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores ... RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely transcribed by RNA Polymerase...

... of the people who participated as mentors or promoters were connected to that Tweet Up in 2013. These...connections brought in folks I didn’t yet know and promotion from those who could not participate but provided...

...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed...

...the site of insertion and which can potentially promote tumorigenesis in vivo. The phenomenon has been ... PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming by transformation-deficient...

...resistance cassettes (Prior et al. 2010), inducible promoters with different degrees of stringency and inducibility...Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli ...

...Cas9 would have an off-target that lands in the promoter of another gene, thereby driving aberrant transcription... said, we generally pick guides by putting the promoter of the gene into a gRNA finder such as WU-CRISPR...

...screening direct interactions between drugs and promoters of oncogenes. He returned to academic research...

...sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to add a ... start of my target sequence? A3: The human U6 promoter prefers a 'G' at the transcription start site ...of the crRNA) is also expressed from a separate promoter. In the pre-processing form, the crRNA contains...

...vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper in PLoS One...