962 results

Use this quick reference sheet for a quick refresher on the basic facts about CRISPR and genome editing.

Jan. 27, 2021, 2 p.m.

Welcome to the Addgene blog! Here you'll find all the info you need on plasmids, CRISPR, viral vectors, fluorescent proteins, science careers, and more.

Jan. 25, 2021, 5:54 p.m.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Jan. 19, 2021, 2:15 p.m.

See the winners from Addgene's 2020 Deck The Lab contest! Contestants could either decorate the lab or make a science-themed winter decoration from home.

Jan. 14, 2021, 1:30 p.m.

Just met some great people while networking? Check out this article for tips to maintain connections and tips for what not to do.

Jan. 12, 2021, 2:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

Find out what were Addgene's most popular blog posts in 2020!

Dec. 29, 2020, 2:15 p.m.

Enjoy 12 new CRISPR articles from Addgene's 12 Days of CRISPR!

Dec. 22, 2020, 2:15 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

An open-source biosensor platform, TRUPATH, measures the association of G protein signal transducers using bioluminescence resonance energy transfer.

Dec. 14, 2020, 2:15 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

Learn about the parts of the AAV RepCap helper plasmid and how they help to package AAV.

Dec. 3, 2020, 2:15 p.m.

Wondering what a software product manger does? Check out this article to see a day in the life of a software product manager in the life sciences.

Nov. 24, 2020, 2:15 p.m.

Discover the history of Rett Syndrome research, the role of the MECP2 gene, and some of the latest breakthrough therapeutic approaches for Rett Syndrome.

Nov. 19, 2020, 2:15 p.m.

Join Addgene for Depositor Week from November 16 to 20, 2020 as we celebrate the role of Addgene depositors in open science!

Nov. 16, 2020, 2:15 p.m.

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

Nov. 12, 2020, 2:15 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

New channelrhodropsins, called ChRgers, avoid intracranial injections and fiber-optic cable implantation for in vivo optogenetics experiments.

Nov. 3, 2020, 2:15 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Find six out-of-the-box ideas for professional development in this virtual world.

Oct. 20, 2020, 1:15 p.m.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Oct. 13, 2020, 1:15 p.m.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Oct. 8, 2020, 1:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Sept. 29, 2020, 1:15 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

Sept. 22, 2020, 1:15 p.m.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

Sept. 17, 2020, 1:15 p.m.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Addgene supports rare disease research with the 2020 BeHEARD Awards. Read about the winners and their research in this blog post.

Sept. 8, 2020, 2 p.m.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Sept. 3, 2020, 12:15 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

Aug. 27, 2020, 1:15 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Aug. 18, 2020, 1:15 p.m.

Addgene marketing intern, Brandon Solis, shares his experience working virtually in a COVID-19 world and his Meet an Addgenie Instagram project.

Aug. 13, 2020, 1:21 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.