We narrowed to 228 results for: cloning
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TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...
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Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m.... passing. We’ve recently piloted Addgene’s new cloning grade DNA service, and viral vector distribution... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are ... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....degraded. Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....basic lab techniques Plasmid Land: The plasmid cloning game ... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....research Browse protocols for molecular biology, cloning, and more ... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....of non-plasmid DNA or heterogeneous and/or mixed clones present in the original sample. At seqWell, we ... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m.... Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m.... To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene of interest... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....after 6 years in the business of western blots and cloning, I decided to pack it in. I had just started month... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector are also available... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....Additional Resources on Addgene.org Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m.... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...