We narrowed to 236 results for: cloning

Take a look at these 5 great apps to make your lab life easier including tools from Gibco, LabArchives, NEB, ThatsMyStapler, and iGreenEarth.

Sept. 22, 2016, 2:30 p.m.

A look at the challenges facing the field of plant synthetic biology, including a discussion of changing public perception, IP concerns, and more.

July 22, 2014, 5:46 p.m.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Feb. 16, 2023, 7:05 p.m.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.

June 29, 2021, 1:15 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Dec. 19, 2024, 2:15 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Oct. 4, 2016, 2:30 p.m.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

May 6, 2025, 1:15 p.m.

Read how the Wu lab is screening over 3,000 antibodies in mice and human brain IHC to verify antibodies for addition to the mAb3D atlas website.

Aug. 9, 2022, 1:15 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

an overview of qPCR and ddPCR copy number variant assays.

April 26, 2022, 1:15 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

July 12, 2018, 1:09 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.