We narrowed to 14 results for: Bacillus

1. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

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   Blog Post

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   Dec. 11, 2017, 2:02 p.m.

2. Easing the Protein Purification Process with pCri

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   Blog Post

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   June 19, 2015, 3:08 p.m.

   ... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...

3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

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   April 4, 2019, 12:53 p.m.

   ...bacterial integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes...ICEBs1, an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID...

4. Plasmids 101: Plasmid Incompatibility

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   Blog Post

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   March 31, 2020, 1:15 p.m.

   ...Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis (Lui et al., 2012, Ni et al., 2008, ...Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility. PLoS ...

5. What's New in CRISPR - Spring 2019

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   Blog Post

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   March 21, 2019, 12:47 p.m.

   ...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...

6. Plasmids 101: Repressible Promoters

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   Blog Post

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   Dec. 20, 2022, 2:15 p.m.

   ... of other organisms, such as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303...

7. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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   Blog Post

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   Aug. 22, 2019, 12:43 p.m.

   ...gene. sacB comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that...

8. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

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   July 18, 2017, 12:41 p.m.

   ...involves cloning cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential...

9. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

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   Sept. 14, 2016, 2:30 p.m.

   ...kit can be used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your...

10. 15 Hot Plasmids from 2017

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    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...libraries The Gross Lab at UCSF recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis...

11. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

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    April 11, 2024, 1:15 p.m.

    ... spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010...Gram-positive  Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A..., Streptomyces lividans, Synechococcus spp., Thiobacillus ferrooxidans, Vibrio salmonicida, Yersinia enterocolitica...

12. Some Like it Hot: Thermostable GeoCas9

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    Blog Post

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    Sept. 14, 2017, 12:40 p.m.

    ... Cas9 protein from the thermophilic bacteria Geobacillus stearothermophilus (GeoCas9). G. stearothermophilus...

13. What's New in CRISPR - November 2021

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    Blog Post

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    Nov. 18, 2021, 2:15 p.m.

    ...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this...

14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ... research has shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma...