We narrowed to 36 results for: CMV
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TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent...constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to the mARG expression...
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AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m.... efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...length to ~4.7kB. If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....and light chain, each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic...(green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al.,... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081...these new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred over 512) ...) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog PostPublishedJan. 10, 2018, 4:13 p.m....protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component of... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression...hybrid mammalian promoter Constitutive Contains CMV enhancer, chicken beta actin promoter, and rabbit... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m.... calcium sensor CMV-mito-GEM-GECO1 and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m.... APEX2 is expressed from a Tet/Shield1 inducible CMV promoter and targeted to a sequence of interest with... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m.... pcDNA3, comes from the pcDNA series. It has the CMV promoter for mammalian expression, a multiple cloning... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression...plasmids: Luc (intronless; CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I...constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or a promoterless construct. Pre-... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....that will be produced. Many common promoters. like CMV, EF1A, and SV40 promoters, are always active and ... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector are also available. These... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust expression post-transfection... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....cells where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids) ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms we see there... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....transfer plasmids have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression) and a polyA...