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We narrowed to 36 results for: CMV

Showing: 1 - 20 of 36 results
  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cotransfect a mixture of 1248 ng of CMV-gvpA-IRES-BFP plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent...constitutively-expressed mARG plasmids driven by CMV promoters. Labs that are new to the mARG expression...
  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...length to ~4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized...
  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...and light chain, each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic...(green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al.,...
  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081...these new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian...
  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred over 512) ...) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from...
  6. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component of...
  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression...hybrid mammalian promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit...
  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... pcDNA3, comes from the pcDNA series. It has the CMV promoter for mammalian expression, a multiple cloning...
  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression...plasmids: Luc (intronless; CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I...constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or a promoterless construct. Pre-...
  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...that will be produced. Many common promoters. like CMV, EF1A, and SV40 promoters, are always active and ...
  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust expression post-transfection...
  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...transfer plasmids have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR...
  13. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...structures (Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression) and a polyA...
Showing: 1 - 20 of 36 results