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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 67 results for: CMV

Showing: 1 - 20 of 67 results

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...length to ~4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized...

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...and light chain, each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic...(green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al.,...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081...these new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred over 512) ...) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression...hybrid mammalian promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression...plasmids: Luc (intronless; CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I...constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression, or a promoterless construct. Pre-...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1 SLC1A3 CMV Episodic ataxia ...DsRed-p150 217-548 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron...moPrP(3F4) D177N PRNP CMV Dementia Susan Lindquist 1321 cytosolic moPrP (3F4) PRNP CMV Dementia Susan Lindquist...FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS Peter Howley 10880...DNMT1 GFP CMV Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 DNMT1 GFP CMV Hereditary...PINK1 Myc CMV Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson...C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark...

  16. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...from the CMV promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed 73349...pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro 73037 Expresses Nluc and copGFP from the CMV promoter pCDH-CMV-Nluc ...Xgal/IPTG plate pAdx-CMV-copGFP 73346 Expresses copGFP from the CMV promoter pAdx-CMV-tdTomato 73347 Expresses...dsRed from the CMV promoter pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter...promoter pCDH-CMV 72265 Express gene of interest from the CMV promoter pCDH-EF1 72266 Express gene of interest...contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ (Addgene #73345) is uniquely suited...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly...Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian... luciferase Ming-Chih Lai 100984 pGL4.18 CMV-Luc Firefly CMV Mammalian expression of firefly luciferase... Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...firefly luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase...Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly luciferase...

  18. Adenovirus Plasmids

    Type
    Collection
    ...Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter Vogelstein... pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene under CMV promoter ...pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV Shuttle...mRFP-trackable viruses containing transgene under CMV Bamburg 50958 ShuttleNSE Shuttle For production of...mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with...
Showing: 1 - 20 of 67 results