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We narrowed to 8 results for: Gateway destination

Showing: 1 - 8 of 8 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...With the appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...attB sites creates attL and attR sites.   Gateway vectors contain modified versions of the att sites...

  2. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...and do not appear in the final construct. The destination vector contains sites with complementary overhangs...ligation can be done in one 30-minute reaction. The destination vector and entry vector(s) are placed in a single...IIS enzyme and ligase. Although the original destination vector + insert may spontaneously religate, this...can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with an attB... to efficiently clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages...inactivation step. If your genes of interest or destination vector contain multiple internal restriction ...want to consider using an alternative method like Gateway cloning or Gibson assembly. Another important consideration...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...ligated into Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt...ordered assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden ...together to allow for ordered assembly into a destination vector that expresses Cas9. This is illustrated...drive ordered assembly into a Cas9-containing destination vector. Note: depending on which method you use...transcriptional activator)-containing plasmids. Each destination vector contains GFP, enabling you to select cells...express up to 7 gRNAs. The kit contains different destination vectors depending on the total number of gRNAs...most gRNA is cloned into the Cas9-containing destination vector. These constructs are digested using BsaI...

  4. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...toxin CcdB. Gateway cloning takes advantage of this property by including ccdB on its destination plasmids...plasmids. In the destination plasmid, the ccdB gene is flanked by recombination sites. To introduce a gene of... of interest into the destination plasmid, recombination sites must first be added to flank the gene. ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...components into a destination vector in a predefined order. Finally, the destination vectors are used ...be used for each Multisite Gateway compatible destination vector for a maximum of 24 elements assembled...component plasmids from building blocks via Golden GATEway Cloning Frustrated by the complexity of assembling...combining the advantages of Golden Gate and Multisite Gateway cloning methods developed by the lab of Joachim...just the set of tools that you need. The Golden GATEway cloning kit simplifies the cloning process for ...used with Multisite Gateway cloning to generate the final construct. Up to eight entry vectors can be ...transgenesis construct. Plasmids constructed using Golden GATEway cloning have been utilized to create recombination...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable...an adapted destination vector with a heterologous promoter outside of the Multisite Gateway® cassette,... systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate ... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...your gene of interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...PMID: 26854857   Examples of FusX-Compatible Destination Vectors Available at Addgene: Lab Plasmids...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...
Showing: 1 - 8 of 8 results