We narrowed to 8 results for: Gateway donor

... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...ccdB gene is excised from the donor vector.   Figure 2: The Gateway system adopts phage integration...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame...coli. Glossary of Gateway cloning vectors Vector type  Vector features Purpose Donor vector attP sites...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...

... the donor is quenched when FRET occurs. By photobleaching the acceptor, you release the donor's quenching...fluorescence decay time of the donor. When FRET occurs between the pairs, donor fluorescence is quenched and...non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means ...orientation will be fixed. The quantum yield of the donor (number of emitted photons per absorbed photons)...signal you should choose the highest quantum yield donor, the highest absorbing acceptor and fluorophores...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... the acceptor with the laser used to excite the donor. In that case the cross-talk is high and your background...

...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)...

...requirements are different for the donor and acceptor fluorophores. The donor must be delivered to an exact... when donor and acceptor are close together. The binding of acceptors >100 Å away from the donor doesn...Let’s consider the donor first where labeling specificity is most important. Donor labeling and the need...for specificity Donor fluorophores need to go to the right place. Mis-targeted donors never “see” FRET...by a sea of uncoupled donors. The best method for targeting a fluorescent donor to your protein is to ...Madani et al., 2009). Donor and acceptors: Putting it all together The FRET donors and acceptors described...table below for donor and acceptor pairs that you can use in your FRET experiments. Donor Acceptor Tag...

...sex pilus, a “bridge” of cytoplasm that allows a donor cell to transfer genetic material to a recipient...also have more than one function: for example, Gateway cloning relies on genes found on an E. coli fertility...contain this F plasmid is critical for successful Gateway cloning. In addition, scientists have engineered...

...example, you may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis...For plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids...

...on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable... systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221...heterologous promoter outside of the Multisite Gateway® cassette, you can specifically express your new... combined in a specified order using Multisite Gateway cloning. A desired insert is cloned using traditional...

...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...