Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
Type
Blog Post
Published
Sept. 26, 2024, 1:15 p.m.
...Ligation-independent cloning
Ligation-independent cloning (LIC) was first developed in the 1990s. While traditional...transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded overhangs can hold a nicked...repairable by the bacteria after transformation.
LIC is a reliable cloning method, but it is limited by...back the entire 10–12 bases. As such, the use of LIC is often limited to specially designed plasmids.
...ligation-independent cloning: A SLIC makeover
In 2007, LIC received an important update, courtesy of Addgene...-independent cloning (SLIC), eliminated many of LIC’s constraints by relying on the power of homologous...