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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 113 results for: LIC

Showing: 1 - 20 of 113 results

  1. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...26094 pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag...cleavage, KanR 26098 pCW-LIC EF460848 No tag, double ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine...AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression... Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with...proteins of medical relevance, and place them in the public domain without restriction. Many SGC plasmids from...cart, then complete the checkout process online. Click here for additional ordering information....

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression...cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression ...bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection page...Trx (thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV ...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...our dedicated Cre-lox Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine...Zebrafish/Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression...mOrange 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression...mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry...Zebrafish/Xenopus/C.elegans/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression...

  4. TALEN Plasmids and Kits

    Type
    Collection
    .... Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Pablo Rivera-Fuentes 111723 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111724 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111725 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111726 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111727 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111728 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111729 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl Arrowsmith 26094 pET28a-LIC T7-lacO Lactose...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...Plasmid Series contains plasmids suitable for replication in many gram-negative bacteria with different...BiFC studies. ID Plasmid Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor...FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc...pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia...Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa...

  7. Open Enzyme Collection

    Type
    Collection
    ...E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...(Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and ...and small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 ...:7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect...sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) ...:4003-4012.e6. Xavier Nicol cyclic di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical...Fluorescent biosensor of the cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with...protein for NADH/NAD+ ratio Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence...

  10. Antibody Guide

    Type
    Collection
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

  11. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...each of the above serotypes and buffers. Please click on the plasmid name for ordering information. ID...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

  14. CRISPR Guide

    Type
    Collection
    ...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: Plan Your Experiment ). In addition to ...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... the original publication below. You can also download the full-text of the publication here . Article...bands from biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with... of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details...single-site small indels given the efficiency of biallelic modification, ease of rapid identification by ... sgRNA located within exons will enrich even monoallelic deletion clones for loss of function. This is...useful to characterize the non-deleted allele in monoallelic deletion clones. For small deletions (as the ...is critical that the deletion and non-deletion amplicons be easily resolved on an agarose gel for multiplexing...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...

  17. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...headings. Click on the publication link to view all other plasmids from that article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ... of applications. To learn more and plan your experiment, check the plasmid's original publication or ...luciferases in our collection include the red and green Click-beetle luciferases ( CBRluc and CBGluc , respectively...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and...Koen Venken 133315 pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring...Daniel Voytas 133312 pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s clear...just as useful (if not more so) for research applications, eclipsing past genome engineering technologies...for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated that... PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve the tool...various species, as well as the development of new applications. The first CRISPR papers described two main ...RNA, making it a very flexible system. Other Applications Scientists have also used the targeting capability...Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease, with the...

  20. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...
Showing: 1 - 20 of 113 results