Molecular Cloning Techniques
Type
Guide
...Independent Cloning (LIC) relies on the 3' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA ...nicks that bacteria repair after transformation. LIC does not require site-specific recombination or a...making it an easy, cheap, and rapid cloning method. LIC depends on the addition of only one free dNTP to ...will repair the nicks. It is important to note that LIC has difficulty assembling DNA fragments with repetitive...more in our SLIC blog post . Figure 6: Summary of LIC. Created with BioRender.com. Yeast-mediated Cloning...