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We narrowed to 18 results for: Streptavidin

Showing: 1 - 18 of 18 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...fluorophore-conjugated streptavidin protein, which binds the primary antibody through the biotin-streptavidin interaction...detection. The advantage of this system is that streptavidin conjugates are commercially available for a ...fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!   ... two-protein labeling by utilizing(a) biotin-streptavidin binding and (b) antibody host species interactions...or protein-protein interactions, like biotin-streptavidin binding and antibody host species reactivity...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... are tagged with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe...biotinylated primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes... concept to lateral flow strips that contain streptavidin which can bind to the biotinylated targets. ...

  3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction allows scientists to...hours biotinylated proteins can be captured on streptavidin affinity beads or matrices. The bait protein...Biotin tagged proteins can then be purified using streptavidin and separated via flow cytometry.  Find plasmids...

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...egg-white protein avidin (or its derivatives streptavidin and neutravidin) is for all practical purposes...purification of biotinylated protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... but it has a strong affinity for avidin and streptavidin, both of which can bind up to four biotin molecules...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and, more recently, OLLAS ...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534–545. PubMed...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation (IP), and tandem mass tag...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...this proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing (BLESS) (Crosetto...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...fused to a streptavidin core that are able to retain reporter proteins fused to the streptavidin-binding ...
Showing: 1 - 18 of 18 results