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We narrowed to 15 results for: Tet-On Advanced

Showing: 1 - 15 of 15 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...2nd generation Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For...gene-function/tet-inducible-expression-systems/tet-systems-legacy-products/tet-on-and-tet-off-2nd-generation...10.1101/gad.2.6.718. PMID: 2843425.Takara Bio. Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com...promoters Tet-Off In its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator...operator sequences (TetO), preventing transcription. In the presence of tetracycline (Tet), TetR preferentially...preferentially binds Tet over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church ...to promoters containing TetO elements (often linked in groups of seven as a Tet Response Element (TRE))...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in...activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo...communication and sharing will only help research advance. Remember: productivity is infectious!  Additional...

  3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...technical developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system...Initiative and how this initiative will lead to advances in human health. It was inspiring to hear how ...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...synthesizing fluorophore fusions on their own. Subsequent advances in fluorescent proteins have replicated many of...sensitivity, photoactivation, etc. These impressive advances lead to an obvious question: In this age of GFP...dye structure have yielded commercial panels of advanced rhodamine fluorophores, such as the Alexa Fluor...properties inside living cells, especially for advanced imaging experiments. We continue to develop derivatives...rather be in the lab making new fluorophores for advanced imaging modalities. Follow him on twitter @rhodamine110...FlAsH, ReAsH) and a short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...organisms. Advances in tissue clearing Similar to light microscopy, there have been significant advances in tissue... beginning of this century has seen some major advances in light microscopy, particularly related to the...in 3D with unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence...spinal cords by combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years ...

  6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy, University of Amsterdam. The previous...Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation APEX suffered...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...still, this challenge did not hinder them from advancing their careers and achieving success.” Pedro Gonzalez...would like to be in a few years, helping me to advance in my career.” Adrian Mancebo (Mentee IMP 2014-...University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees held scheduled...the International Mentoring Foundation for the Advancemnet of Higher Education website Resources at Addgene...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... learners and have individual priorities for advancement in science Have their own working schedules and...managing people: Skills for New Managers by Morey Stettner Successful Manager's Handbook: Develop Yourself...

  10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...areas of biological research and have been key in advancing our knowledge in areas such as bacterial conjugation...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...

  11. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...immunity with exosome-enveloped AAV vectors." Blood advances 1.23 (2017): 2019-2031. PubMed PMID: 29296848....Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...dual-plasmid approach makes it a valuable resource for advancing our understanding of its biology, pathogenicity...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins in living cells... nonfluorescent until they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that can be inserted ...interest, resulting in a C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small...et al. "Hairpin structure of a biarsenical− tetracysteine motif determined by NMR spectroscopy." Journal...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...gene expression studies, which are evinced by the Tet system and visualization of single molecules via ...Vale lab and the Weissman lab have teamed up and advanced technology in the fields of single molecule imaging...NLS-MS2-RFP) the reporter mRNA, which contains the tethering sites for the fluorescent proteins and an inducible...however, remain bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA...MS2 bacteriophage coat protein binding sites to tether many copies of the FP-coat protein fusions to their...
Showing: 1 - 15 of 15 results