We narrowed to 15 results for: ccdB

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Jan. 28, 2014, 3:10 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

In this blog post, learn how Addgene tracks samples and how you could implement a similar system in your lab.

Oct. 6, 2015, 2:30 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.