We narrowed to 84 results for: control gRNA

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Jan. 10, 2017, 3:37 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

Oct. 20, 2022, 1:15 p.m.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Sept. 3, 2020, 12:15 p.m.

Learn more about Addgene's NGS verification process for pooled libraries using real-life amplication data from the Brunello gRNA library.

Oct. 26, 2017, 1:59 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

Feb. 8, 2024, 2:15 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

New CRISPR pooled libraries from the Broad Institute allow you to specifically target the mouse and human kinomes in your next screening experiment.

Sept. 9, 2016, 2:43 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

March 10, 2020, 1:15 p.m.

Find out what CRISPR tools are distributed as a part of Addgene's new viral service.

Dec. 6, 2016, 3:30 p.m.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

Aug. 10, 2017, 2:19 p.m.