Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
Type
Blog Post
Published
Oct. 11, 2018, 12:30 p.m.
...Golden gate assembly limitations
Embraced by the synthetic biology community, Golden Gate Assembly is commonly...in-frame assembly proceeding in 99% of 12-fragment assemblies and over 90% for 24-fragment assemblies (Figure...any Golden Gate assembly design.
Number of fragments assembled
Golden Gate Assembly protocol* (Volume...from 2 µl of the assembly reaction, and from the entire assembly reaction. All assembly reactions had a... 20+ fragment assemblies with both robust efficiency and accuracy.
Golden Gate assembly involves the ...enhancing the ordered assembly of the target construct.
Development of Golden Gate Assembly test systems
Our...different levels of assembly:
High efficiency and accurate single-insert assembly
Intermediate 5- or ...