Fluorescent Proteins 101: Luciferases
Type
Blog Post
Published
July 22, 2025, 1:15 p.m.
...expressing luciferase
Luciferase reporter assays for monitoring gene regulation
Since firefly luciferase was ...Well-established
Natural and engineered luciferases
Although firefly luciferase was the first bioluminescent reporter...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options.
Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced... single protein.
Considerations for luciferase assays
Luciferases can only produce light in the presence...