FlipGFP, a novel fluorescence protease reporter to study apoptosis
Type
Blog Post
Published
May 21, 2019, 12:10 p.m.
...reporter based on superfolder Cherry (sfCherry). Like FlipGFP, sfCherry was split into β1-9 and β10-11, where...cleave proteins. This protease reporter based on sfCherry however did not initially fluoresce in HEK293 ...first improved the self-assembling capability of sfCherry approximately 20 fold by directed evolution. They...