...development. Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially...biology that arose from zebrafish-based investigations. Genetic resources for zebrafish A common approach ...implemented in zebrafish. Developmental biology insights using lineage tracing in zebrafish To highlight...control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside of...Friedrich Miescher Lab of the Max Planck Society. Zebrafish (Danio rerio) have been used since the 1930’s ...maintain. Also in contrast to mice, the transparent zebrafish embryo develops outside of the mother, making ...embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism, I’ll outline...

...et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5 days post ...a high degree of control over time and space. Zebrafish have become a popular model organism because their...well to studies of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live...short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins which are light sensitive...to vertebrates and are commonly introduced in zebrafish through the UAS/Gal4 system for behavioral studies...by acoustic, visual or tactile stimuli, larval zebrafish react by performing a distinct C-bend to escape...

...compounds in one experiment. Zebrafish (Danio rerio) Since the 1960s, the zebrafish has gradually become an...with a disease have a homolog in zebrafish (Howe et al., 2013). Zebrafish are small, easily maintained as...easily bred, and produce 50-300 eggs at a time. Zebrafish embryos are also laid and fertilized externally...their genomes or to create transgenic animals. Zebrafish are an ideal model organisms for embryonic development...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using ...PMCID: PMC2736433. Howe, Kerstin, et al. "The zebrafish reference genome sequence and its relationship...

...targeting the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids...Gateway-Compatible Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases...

..., generate a dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created..., termed BLITZ (Biotin Labeling In Transgenic Zebrafish) (Xiong, et al., 2021).    Fig. 1: Schematic...be co-expressed in essentially any transgenic zebrafish line expressing a GFP-tagged version of a protein... BLITZ system was designed and optimized with zebrafish in mind, it can easily be applied to other systems...

...cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos and adult fish. Zebrafish are ...experimental settings: HEK293 cell culture and in live zebrafish. For cell culture experiments, only dL5**-expressing....  The video below shows what happens to the zebrafish’s heartbeat and blood circulation of following ...

...stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic zebrafish lines that allow for...(Hadjieconomou et al., 2011). Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow...(Pan et al., 2011), Brainbow was also used in zebrafish by injecting a CMV-Brainbow construct at the embryonic...Albert, et al. "Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): ...

...and lineage tracing in other systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights... study model organisms such as Drosophila and zebrafish. Brainbow in Drosophila has aided in the mapping...motor neurons and the neuromuscular junction. In zebrafish, the method has become very useful in lineage ...labeling for cell tracing and lineage analysis in zebrafish. Pan YA, Freundlich T, Weissman TA, Schoppik D...

...experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse brain slices and freely moving mice...threatening looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course...

...developed the LipoGlo system in zebrafish (Thierer et al, 2019). Zebrafish are a great model for high throughput...

...roles of three Rab proteins during a key stage of zebrafish development (Aljiboury et al., 2023). Rab family...be difficult to unravel their specific roles. Zebrafish are an excellent model for optogenetic studies...Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. PLoS Genetics...

...used FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells..., a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal...

...CreLite for cell tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express...found in both deep and superficial tissues of zebrafish studied, such as the brain, eye, muscle and epithelial...

...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880...allow scientists to quickly and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative...in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting...

...systems, including E. coli, plants, Drosophila, and zebrafish!   Why use multiplexed gRNAs? By expressing multiple...system, check out this blog post.  Multiplexing in zebrafish Wenbiao Chen Lab Golden Gate Assembly Multiplex...These plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending...

...other species such as non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics...Wyart. "Optogenetics: a new enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3...

...five model organisms: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox...

...predicted to target sites in the human, pig, or zebrafish genome, this system can be used in these organisms...

...organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent...

...mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not thermophilic (high...