We narrowed to 10 results for: Free Use GFP

...high concentration. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all...AcGFP1-C1 - Mammalian Expression sfGFP (superfolder GFP) 485 507 54 14 min Prone to dimerization sfGFP-N1...dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral...A206K) cfSGFP2-N - Mammalian Expression (cysteine-free SGFP2) ZsGreen 493 505 39 Tetramer pHIV-Zsgreen ... excitation and emission maxima (Stokes shift), useful for applications like multicolor imaging with better...Brightness pKa Maturation Structure Plasmids Free Use GFP (fuGFP) 395 503 Monomer pUS252 - Mammalian Expression...Expression mPA-Emerald-C1 - Mammalian Expression PA-GFP 504 517 400 14 Monomer (A206K) pPAGFP-N1 - Mammalian...

...Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev...lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for...controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression systems...encode tools for affinity purification (which can be used for viral TRAP). ID Name Expression System Activity...Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments, including neuronal...Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals...

... titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR...Endotoxin contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources Browse...Overview of Viral Production Viruses are generated using standard methods that have been optimized for each...Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding Rep and...facility that produced the viral vector lot. Contact us to determine which facility produced your viral vector...

...available at Addgene for use in worms. Browse Plasmids Plasmid Tools for Use in Worms The table below ... repository? Help us widen our resources for the community by depositing your useful tools , or by suggesting... a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that expressed...as CRISPR and synthetic biology collections) for use in C. elegan research. Plasmid...Caenorhabditis elegans ( C. elegans or “the worm”) is widely used as a genetic model organism due to their rapid life...genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...below highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones...

...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...generated with either phosphoserine using SepOTSλ (Plasmid #68292) or serine using supD tRNA ( SupD (Plasmid #68307... production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications ,...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These...RSF1030 origins or a low copy p15a origins should be used. Find further details in Mohler et al. (2023) and...either side occurring within the parent protein. Using the recoded strain of E. coli , C321.ΔA (Bacterial...

...proteins by using donor constructs that harbor synthetic short homology arms. Notably, they have used Auxin-...developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression...marker-free mutations, insertions, and deletions at any locus in C. elegans . This system uses a 10-day...as well as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post...information about HDR/repair template design. How to use CRISPR to tag your gene of interest Mendenhall and...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid...

...split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC...split-luciferase complementation assay in plants using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-...luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed...emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...enhancers using a firefly luciferase reporter. These plasmids improve on the original STARR-seq by using the...luciferase have short protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal...Firefly Insertion of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly...

...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...more about the components of tet systems and how to use them, or skip ahead to view highlighted Tet plasmids... interest should only be expressed occasionally, use Tet-On: express an rtTA or TetR and include a tet-responsive...generally active and only turned off occasionally, use Tet-Off: express a tTA and include a tet-responsive...upstream of your gene of interest. Many plasmids use "TetO" or "Tet-O" in their name or description, meaning...or Tet-On systems, depending on whether they are used alongside a tTA or rtTA, respectively. You should...

...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...negative-control constructs used to characterize the sensor's behavior in the target system. Use care when applying...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...browse all the biosensors tagged in our catalog . Use the article links to find the plasmids associated...associated with the article. We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...intraorganellar Ca 2+ at subcellular resolution using CEPIA. Nat Commun. 2014 Jun 13;5:4153. Masamitsu...

...multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large...these PAM-flexible Cas9s are useful for precision edits, they can be used for virtually any CRISPR application...libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from...concentration too low to be used in experiments and must be amplified before use (Figure 8C), following the...will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for screens...repression using a TET1-dCas9 fusion. Figure 10: Overview of epigenetic modification using CRISPR. Browse...co-opted for use in eukaryotic cells . The Cascade complex is recruited to target DNA using a gRNA and ...