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We narrowed to 12 results for: Free Use GFP

Showing: 1 - 12 of 12 results

  1. Viral Production

    Type
    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...Endotoxin contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize...addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources Browse... titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR...Resources Overview of Viral Production Viruses are generated using standard methods that have been optimized...of unexpected DNA contaminants. The data is also used to determine recombination rates in FLEX/DIO/fDIO...protein present in the sample is also determined and used to determine purity of the AAV preparation. Figure...

  2. Worm Expression Resources

    Type
    Collection
    ...available at Addgene for use in worms. Browse Plasmids Plasmid Tools for Use in Worms The table below ... a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that expressed... repository? Help us widen our resources for the community by depositing your useful tools , or by suggesting...as CRISPR and synthetic biology collections) for use in C. elegan research. Plasmid...Caenorhabditis elegans ( C. elegans or “the worm”) is widely used as a genetic model organism due to their rapid life...genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...below highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG... production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications ,...generated with either phosphoserine using SepOTSλ (Plasmid #68292) or serine using supD tRNA ( SupD (Plasmid #68307...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These...RSF1030 origins or a low copy p15a origins should be used. Find further details in Mohler et al. (2023) and...library (Pooled Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression...marker-free mutations, insertions, and deletions at any locus in C. elegans . This system uses a 10-day...proteins by using donor constructs that harbor synthetic short homology arms. Notably, they have used Auxin-...information about HDR/repair template design. How to use CRISPR to tag your gene of interest Mendenhall and...Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid...cell lines. To repeat the tagging in your system, use the plasmids as listed in each row. If your own gene... and right homology arms. More details on how to use this plasmid in your experiments can be found below...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Brightness pKa Maturation Structure Plasmids Free Use GFP (fuGFP) 395 503 Monomer pUS252 - Mammalian Expression...Expression cfSGFP2 493 517 34 5.4 Monomer (A206K) cfSGFP2-N - Mammalian Expression (cysteine-free SGFP2) ZsGreen...Mammalian Expression sfGFP (superfolder GFP) 485 507 54 14 min Prone to dimerization sfGFP-N1 - Mammalian Expression...Expression EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Expression PA-GFP 504 517 400 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian... pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 ...high concentration. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...split-luciferase complementation assay in plants using Gateway cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC...split-luciferase complementation assay in plants using Gateway cloning. Use with 174050 Alberto Macho 136010 psi-...enhancers using a firefly luciferase reporter. These plasmids improve on the original STARR-seq by using the...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...luciferase have short protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...more about the components of tet systems and how to use them, or skip ahead to view highlighted Tet plasmids... Tetracycline On (Tet-On) In 1995, Gossen et al. used random mutagenesis to identify the amino acid residues... interest should only be expressed occasionally, use Tet-On: express an rtTA or TetR and include a tet-responsive...generally active and only turned off occasionally, use Tet-Off: express a tTA and include a tet-responsive...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus... 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...neuronal tracing, to be used with modified rabies virus. Viral...virus-based monosynaptic tracing. These AAV can be used with modified rabies virus to perform retrograde...coated RAVB is released from the starter cell and free to infect neighboring cells via G-protein receptors...viral vector packaging service, but lets us know what viruses would be helpful to the scientific community...

  9. Genetic Code Expansion

    Type
    Collection
    ...first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You should also...to be allocated to the new amino acid. Because there are no free codons, this can be challenging. In E....Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...amber stop codon (UAG) and thus this codon is often used. The gene of interest can be expressed from a plasmid... observed. The introduced amino acid can also be used to intentionally change the activity of a protein...growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal pairs of...unallocated codon. Therefore many controls must be used to make sure that these conditions are true. Always...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...negative-control constructs used to characterize the sensor's behavior in the target system. Use care when applying...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...browse all the biosensors tagged in our catalog . Use the article links to find the plasmids associated...associated with the article. We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...Fluorescent Mg2+ sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes...

  11. Antibody Production

    Type
    Collection
    ...antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is first transiently...not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before storing...Addgene have been produced in-house by Addgene scientists. They are generated using standard methods individually... sodium azide. This buffer is not compatible for use in live cells and will interfere with conjugation...our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient Instructions...for 7 days and then purified from the supernatant using Protein A or Protein G affinity columns. Formulation...chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer...

  12. CRISPR Guide

    Type
    Collection
    ...these PAM-flexible Cas9s are useful for precision edits, they can be used for virtually any CRISPR application...concentration too low to be used in experiments and must be amplified before use (Figure 8C), following the...libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from...co-opted for use in eukaryotic cells . The Cascade complex is recruited to target DNA using a gRNA and ...bacterial genomic region used in pathogen defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator...multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large...will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for screens...
Showing: 1 - 12 of 12 results