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We narrowed to 22 results for: SAM activators

Showing: 1 - 20 of 22 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library SAM can ...please visit the Addgene SAM library page . Targeted gene activation using SAM There are two sets of 3 ...references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR... available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with... Return to GeCKO Library SAM Library Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

  2. Plan Your Experiment

    Type
    Collection
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes...Knockouts Activation/interference Needs good primer design Western blot Knockouts Activation/interference...the gRNA activity. Be sure to sequence the genomic region you intend to target in your samples to ensure...proteins) Many base editors fused to Cas9 exert their activity within a very narrow window proximal to the PAM...dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently...essential protein domain could abolish protein activity and essentially function as a “knockout,” but ...

  3. Luciferase Plasmid Collection

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    Collection
    ... has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α ... upon interaction, the NanoBiT subunits form an active enzyme, generating luminescence. Find plasmids ...and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers...G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live imaging of mTOR...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* Ulrik Gether 119741 AAV SYN flex PSAM4 GlyR IRES...chemogenetics AAV to chemically induce neuronal activity in specific cell types. See our Chemogenetics ...for more information about this field. Receptor Activating hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory HCAD...mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent...Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361...44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg...pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...light that activates the opsin to either excite or inhibit the target neuron. LMO can be activated either ...Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...(Gq) and rM3D(Gs) are activating, while hM4D(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic...types of PSAMs: 5HT3 coupled, which are activating, and GlyR-coupled, which are inhibitory. Another recently...encode genetically engineered receptors that are activated by specific small molecules, allowing a scientist...expressed, these receptors can be used to artificially activate or inhibit specific biological processes. Addgene's... activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types ...

  6. CRISPR Guide

    Type
    Collection
    ...nuclease activity Cas9 enzymes will likely improve as more key residues for specificity and activity are discovered...NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity SpRY — NRN/NYN, where R = A...select for gRNAs with high on-target activity and low off-target activity. Knockout libraries often target...become a flexible tool to activate, repress, visualize, and isolate genes. Activation or Repression of Target...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; Figure 9A). Other examples of activation systems...instead of a two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified...additional RNA-binding helper activators; highest levels of single-gene activation (Figure 9D) Figure 9: Overview...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... BbsI yes, activate, N-terminal S. pyogenes Zhang PX856 62888 Mammalian BbsI yes, activate, C-terminal...Bacteria BsaI yes, activate S. pyogenes Marraffini pWJ68 46571 Bacteria BsaI yes, activate S. pyogenes Marraffini...yes, activate S. pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from pXL yes, activate S. ... none S. pyogenes Boxem lentiSAMv2 75112 Mammalian/Lentiviral U6 yes, activate S. pyogenes Blast Zhang...Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting...-in-one vector, but their function (cut, nick, activate, interfere, etc.) is limited to that of the Cas...Cas9. We have plasmids with Cas9 that can cut, activate, interfere, or nick. Selection , such as Puromycin...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...signal the presence of specific biomolecules, the activity of specific genes and cellular processes, or other...) High-performance calcium sensors for imaging activity in neuronal populations and microcompartments....GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300. Douglas ...Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci. 2012 Oct 3;32(40):13819-40...genetically encoded calcium sensor Imaging neural activity in worms, flies and mice with improved GCaMP calcium...fluorescent calcium ion indicator for imaging neural activity. bioRxiv 2025.07.28.667269. Alexander Lohman Calcium...

  9. Bacterial Expression Systems

    Type
    Collection
    ...particular protein can activate expression from that element. When this activation requires a transcription...Expression Species PI 14474 pRU1161 Promoter activity GUS activity and fluorescence (mRFP1) Gram-negative bacteria...bacteria Philip Poole 14473 pRU1156 Promoter activity GUS activity and fluorescence (GFPmut3.1) Gram-negative...Promoter activity Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence...Promoter activity Fluorescence (mCherry) Mycobacterium sp. Tanya Parish 24657 pASTA3 Promoter activity Fluorescence...Purification To study the function, structure, and activity of a protein it is generally necessary to purify...engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases. Addgene Blog...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Scientists have adapted these systems to activate/inactivate genes when a recombinase enzyme is present...similarity and the amino acid residue used in the active site. PhiC31 integrase, derived from a Streptomyces...estrogen receptor (ERT2) so that the enzyme is only activated in the presence of tamoxifen. A tetracycline-regulated...Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing...Depending on the construct, gene expression may be activated or repressed. Search by recombinase (Cre, Flp,...) If the target sites are on the same DNA strand (in cis) in the same direction, the sequence between ...orientation. A) If the target sites (loxP) are on the same DNA strand in opposite directions, recombination...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    .../Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this they have taken...sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic...Plasmids 175571 and 175572 express dCas9-VPR for activation. Usage Guide sequences can be designed using ... a unique enhancer and either Ascl1 or Neurog2 activity . Development, 148(12). doi: 10.1242/dev.199399...sequence: Up to two guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain). For ...tetracycline-controlled transactivator (tTA) was created by fusing TetR with the activation domain of the herpes...stronger expression of the downstream gene via its activation domain than simply removing a repressor. Tet-... to doxycycline and three copies of a minimal activation domain from VP16 for increased induction of gene...further improved rtTA variants with lower background activity and greater sensitivity to doxycycline (Zhou et...interest. If your gene of interest should be generally active and only turned off occasionally, use Tet-Off: ...

  13. Antibody Guide

    Type
    Collection
    ...fluorophores are available in a range of colors activated by different wavelengths, allowing for easy differentiation...conjugated with HRP. Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane...antibody, followed by incubation and wash steps. Activate the conjugate and read the output via a plate ... antigen and the conjugate, typically HRP, is activated and its output measured. This method can be performed...secondary antibody is added. The conjugate is activated and the output is read. A standard curve with ...deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry ...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...

  14. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PLAU plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor...mitogen-activated protein kinase kinase 1 MAPKK1, MEK1, MKK1, PRKMK1 MAP2K2 mitogen-activated protein ...mitogen-activated protein kinase kinase kinase 14 FTDCR1B, HS, HSNIK, NIK MAP3K8 mitogen-activated protein...Cdc42/Rac)-activated kinase 1 MGC130000, MGC130001, PAKalpha PAK2 p21 protein (Cdc42/Rac)-activated kinase... protein (Cdc42/Rac)-activated kinase 4 - PAK6 p21 protein (Cdc42/Rac)-activated kinase 6 PAK5 PAK7 p21...the immune system triggers several processes to activate cells to recognize and get rid of infectious pathogens...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... at specific loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors can modulate...processed into individual crRNAs by a special trans-activating crRNA (tracrRNA) with homology to the short palindromic...for gene knockout, as well as transcriptional activation or repression. Combined with the power of next-generation...gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9...eSpCas9, and HypaCas9 display very low off-target activity due to rationally designed mutations. Researchers...CRISPR-Mediated Control of Gene Repression and Activation. Cell . 159(3):647-6. PMID: 25307932 Gilbert ...targeting multiple loci can be easily expressed in the same cell or organism. The ability to precisely target...

  16. Rett Syndrome

    Type
    Collection
    ...transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation, and more. How ...and aggressive plan to use gene editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA ...syndrome can be inherited. In females, random X inactivation results in approximately half of cells expressing...expressing mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2...variability in symptoms even within patients with the same mutation, the location and type of MECP2 mutation...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link opens in a...

  17. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...interactions can be discovered by fluorescence-activated cell sorting (FACS). The Mode #2 phosphosite library... a program of OPTN/NDP52 recruitment and TBK1 activation to promote mitophagy . Molecular Cell , 60 (1...Szeligowski, R. V., Rogulina, S., Gerstein, M., Sampson, J. R., Isaacs, F. J., & Rinehart, J. (2018). Encoding...

  18. Distribution to Industry

    Type
    Collection
    ...Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein Purification...partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're...allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer Information...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... reporter plasmid with very low basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors... Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast...

  20. Brain Initiative Collection

    Type
    Collection
    ...H134R mutation) fused to eYFP for optogenetic activation Viviana Gradinaru 128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn...eYFP Viviana Gradinaru 119741-AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid ...plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid Scott Sternson... about our antibodies ID Recombinant Antibody Reactivity Recommended Applications PI Additional Resources...
Showing: 1 - 20 of 22 results