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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9 results for: Tight TRE

Showing: 1 - 9 of 9 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ... of interest. Tight TRE promoter or P Tight : reduced background expression and tighter binding of Tet-Advanced...gene of interest. tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled... is unable to bind the TRE. In the presence of tetracycline, rtTA binds the TRE, promoting gene expression...for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector...cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521 pCW57.1-MAT2A Lentiviral Tet-Off...Tet-controlled expression (On or Off) None Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for ...and EGFP. Pbi contains a TRE between two minimal CMV promoters. None Pbi (TRE, miniCMV) Bert Vogelstein...

  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... 87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP...AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...reducing cell stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...

  4. Cre-lox system

    Type
    Collection
    ...Stainier 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-...Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre fused to the...GFP and Cre CMV Lentiviral Lu 118029 pAAV-TRE-dDIO-vCre VCre TRE AAV Luo 119063 pBac(3xP3-gTc‚Äôv; Tc-hsp68...CAG Mammalian Zhang 68477 pTRE:iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP...to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The...Cre-containing Plasmids Scientists have developed ways to tightly control Cre expression and to optimize Cre expression...often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yannick Doyon 214916 TRE-TDP-43ΔNLS-mClover3 TARDBP mClover3 TRE ALS Ophir Shalem 214917 TRE-mClover3-TDP-43ΔNLS...pEHA1628) SNCA Avi TRE Parkinson's Vikram Khurana 210360 TMEM106B-2Strep TMEM106B Strep CMV, T7 Frontotemporal...Flag CMV ALS Yossi Shiloh 34927 pTRE_tau-LacZ::tTA-H100Y MAPT LacZ TRE Parkinson's, FTD Mark Mayford 35000...telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart 44333 pMX_mTBP TBP LTR ...Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 ...Dent 188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off...Tet-off Full length Tau (0N4R, P301L/V337M) MAPT TRE Parkinson's, FTD Franz-Ulrich Hartl 188784 pBMN mEGFP-OPTN...

  6. Brain Initiative Collection

    Type
    Collection
    ...the hSyn1 promoter Viviana Gradinaru 117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper... through this effort will provide novel ways to treat, cure, and even prevent brain disorders. The BRAIN...

  7. AAV for Neuronal Tracing

    Type
    Collection
    ...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a specific ...

  9. Antibody Guide

    Type
    Collection
    ...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...creates variety in the antibody allowing for their extreme specificity occurs in the B cell itself prior to...Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope specificity...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ...isolate specific proteins or protein complexes for downstream analysis. They are powerful tools for understanding...Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations One type of ...
Showing: 1 - 9 of 9 results