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We narrowed to 6 results for: gibson assembly

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  1. Molecular Cloning Techniques

    Type
    Guide
    .... Read more in our Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created with BioRender.com...topoisomerase I. Created with BioRender.com. Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more...more commonly known as Gibson assembly, takes advantage of the properties of three common molecular biology...5' exonuclease, polymerase, and ligase. In Gibson assembly, DNA fragments with 20–40 base-pair homology...dsDNA. A major benefit of Gibson cloning is that it allows for the assembly of multiple fragments of DNA...backbone. A drawback of this system is that Gibson assembly works best when combining DNA fragments over...digest Plasmid cloning by PCR DNA ligation Gibson assembly Ligation independent cloning Addgene Blogs ...

  2. Modular Cloning Guide

    Type
    Guide
    ...subunits are first assembled by Golden Gate cloning and then combined by Gibson Assembly into acceptor vectors...modules. In the first assembly step, compatible Level 0 parts are directionally assembled into a Level 1 vector...MetClo DNA Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA...transfections. PUF Assembly Kit Mammalian Expression, Bacterial Expression Huimin Zhao Plasmids to assemble custom-made...pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System Kit Genome Engineering...Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts...Cloning (or MoClo) is a system for efficiently assembling multiple DNA parts into functional plasmids. ...

  3. Adenovirus Guide

    Type
    Guide
    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...primarily encode for structural proteins needed to assemble icosahedral capsids and build new virions. Figure...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...and Cap regions necessary for the production and assembly of viral capsids. Helper plasmid — containing ...rAAV vector (∼5%). Gene fragments can also be reassembled via homologous recombination, when both sequences...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have...
Showing: 1 - 6 of 6 results