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Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Protocols for plasmid cloning by PCR.

Get started with Gibson Assembly Cloning!

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for Ligation Independent Cloning

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Tips on designing primers for PCR

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Best practices for performing sequence analysis of a plasmid

Learn how to add antibiotic to a plate after it's already been poured and solidified.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Follow this protocol to perform RNA extraction without a kit.

In this protocol you will learn the basics of how to weigh reagents.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.