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Platinum Gate TALEN Kit
(Kit # 1000000043 )

Depositing Lab:   Takashi Yamamoto

This kit enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with one of two types of TALE scaffold: +136/+63 or +153/+47. The kit contains 35 plasmdids: 16 module plasmids with non-RVD variations, 11 intermediate arrays, and 8 destination vectors.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs

Add to Cart
$400 USD + shipping

Available to academics and nonprofits only.

Original Publication

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T. Sci Rep 2013 Nov 29;3:3379. doi: 10.1038/srep03379. PubMed .

Description

The Platinum Gate TALEN kit enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with one of two types of TALE scaffold: +136/+63 or +153/+47. The kit contains 16 module plasmids with non-RVD variations, 11 intermediate arrays, and 8 destination vectors.

These Platinum TALENs have been proven to work well in:

Overview of TALEN assembly

Illustration of Platinum Gate TALE assembly

Figure from Sakuma et al., Scientific Reports 2013 Nov 29;3:3379

Protocol

Download a PDF Protocol for TALEN construction using the Platinum Gate TALEN Kit as described in the original publication.

Icon Platinum Gate TALEN assembly protocol (376.4 KB)

Resources

Download a ZIP file containing all Platinum Gate TALEN plasmid sequences in GenBank format

Icon Platinum Gate TALEN Kit plasmids - GenBank files (128.0 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The TALEN kit used for constructing Platinum TALENs was a gift from Takashi Yamamoto (Addgene kit # 1000000043)"

For your Reference section:
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T. Sci Rep 2013 Nov 29;3:3379. doi: 10.1038/srep03379. PubMed .

Platinum Gate TALEN Kit - #1000000043

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 p1HD
Ampicillin
A / 2 p1NG
Ampicillin
A / 3 p1NI
Ampicillin
A / 4 p1NN
Ampicillin
A / 5 p2HD
Ampicillin
A / 6 p2NG
Ampicillin
A / 7 p2NI
Ampicillin
A / 8 p2NN
Ampicillin
A / 9 p3HD
Ampicillin
A / 10 p3NG
Ampicillin
A / 11 p3NI
Ampicillin
A / 12 p3NN
Ampicillin
B / 1 p4HD
Ampicillin
B / 2 p4NG
Ampicillin
B / 3 p4NI
Ampicillin
B / 4 p4NN
Ampicillin
B / 5 ptCMV-136/63-VR-HD
Ampicillin
B / 6 ptCMV-136/63-VR-NG
Ampicillin
B / 7 ptCMV-136/63-VR-NI
Ampicillin
B / 8 ptCMV-136/63-VR-NN
Ampicillin
B / 9 ptCMV-153/47-VR-HD
Ampicillin
B / 10 ptCMV-153/47-VR-NG
Ampicillin
B / 11 ptCMV-153/47-VR-NI
Ampicillin
B / 12 ptCMV-153/47-VR-NN
Ampicillin
C / 1 pFUS2_a1a
Spectinomycin
C / 2 pFUS2_a2a
Spectinomycin
C / 3 pFUS2_a2b
Spectinomycin
C / 4 pFUS2_a3a
Spectinomycin
C / 5 pFUS2_a3b
Spectinomycin
C / 6 pFUS2_a4a
Spectinomycin
C / 7 pFUS2_a4b
Spectinomycin
C / 8 pFUS2_b1
Spectinomycin
C / 9 pFUS2_b2
Spectinomycin
C / 10 pFUS2_b3
Spectinomycin
C / 11 pFUS2_b4
Spectinomycin
Data calculated @ 2017-05-26

Kit Plate Map - #1000000043

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