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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene. A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3' to this insert. Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae & Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814). The URA3 marker is from S. cerevisiae but complements the ura4 allele from S. pombe. Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Schizosaccharomyces pombe, E.coli, S.pombe, Saccharomyces cerevisiae, Schizosaccharomyces pombe SP63. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number