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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Created by Moore, July 1995, under contract with NCBI. Integration vector permitting positive selection for inserts. It can be propagated in a lacI+ strain such as C600 in the absence of IPTG, but replication is inefficient.  Transcription of the replication primer RNA is regulated by the lacZpo promoter/operator. In the presence of lacIq, replication is entirely dependent on the presence of inducer.  The repressible replication system permits temporary maintenance of the plasmid, construction of strains with stable integrants of vector derivatives, and recovery of sequences adjacent to cloned fragments. Can be selected with 100 ug/ml ampicillin and/or 75 ug/ml spectinomycin. Permits positive selection because deletion of the aadA fragment is not viable.  pAM35 (ATCC 77186) differs from pAM34 (ATCC 77185) by deletion of the DraII fragment in pAM34 carrying lacIq.  E. coli JS219 is MC1061 malPp delta534::lacIq.  The order of the major features in this plasmid is: lacZpo - ori - rom - transcription terminator - BamHI - MCS - XmaIII - aadA (spcR) - HindIII - MCS - BamHI - transcription terminator - bla.  Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--3.0, 1.8; KpnI--3.4, 1.4. (ATCC staff) Medium is -1 1227 + IPTG (1 mM). Hosts: E.coli JS219, E.coli. Related vectors: pAM34. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- M69063, X07465, X04395