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Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pBC12BI


Plasmid Type
Cloning Method
cDNA clones without an initiation codon may be cloned in frame into the unique BamHI site 3' to the AUG of the rat preproinsulin gene. This results in 5 - 6 additional amino terminal amino acids from the insulin signal peptide. [1] Genomic sequences are normally inserted between unique HindIII and SmaI sites (5' to 3'). This deletes the preproinsulin gene from the vector. [1] cDNA sequences with a translation initiation codon are usually inserted between the unique HindIII and BamHI sites (5' to 3'). [1] Polyadenylation signals (and possibly carboxy terminal amino acids) are contributed by the preproinsulin gene. [1] This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the BamHI and ClaI sites). [2] Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--2.8,0.78,0.44; HindIII--4.1; BstEII--4.1. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli RR1, vertebrate cells, E.coli, monkey COS-1 cell line, mammal cell line, avian cell line. (Information source: VectorDB ( ).)
Catalog Number