Vector Database

Size is 4363 for Boehringer, BRL, Promega, Sigma. Size is 4362 for USB. Promoter P1 is artificially created by the ligation of two different DNA fragments to create pBR322. Promoter P2 in the same region as P1, but it is on the opposite strand and initiates transcription in the direction of the tetracycline resistance gene. Promoter P3 is the natural promoter for the beta-lactamase gene. Medium is 1227 LB plus ampicillin. The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases first through the tet genes, the pMB1 material, and finally through the Tn3 region. NCBI gi: 58257 Hosts: E.coli HB101, E.coli C600, E.coli RR1, E.coli. Related vectors: pSC101. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.) Also, from Bolivar et al., Gene, 77: pBR322 is derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The antibiotic-resistant genes on pBR322 are not transposable. The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and SalI.