Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHSREM1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3400
- Notes
- A KpnI/SacI double digest releases the hsp70 transcription module, for subsequent ligation into other plasmids such as ones containing Drosophila P elements. KpnI BglII NotI XhoI - promoter - MCS - terminator - SpeI XbaI NotI EagI BstXI SacII SacI. One of a series (ATCC 37642-37645) of plasmid vectors for expressing genes under the control of the Drosophila hsp70 promoter & terminator. The transcription module is flanked by restriction sites (facilitating its removal), T7 and T3 promoters (for in vitro RNA synthesis), & primers for Sanger sequencing. There is also an M13 origin of replication. Restriction digests of the clone give the following sizes (kb): PstI--3.4; HindIII--3.3; EcoRI--3.4; BamHI--3.3. (ATCC staff) BamHI/BglII fragment of pCIII1 with terminator plus several upstream sites of Bluescript MCS ligated into BamHI of pCII38 = pCIII8. KpnI/XhoI region of pCIII8 replaced with synthetic adapter with NotI and BglII sites = pHSREM1. (personal communication) XhoI/HindIII fragment of Drosophila hsp70 with nt -89 to +89 (relative to transcription start site) ligated to XhoI/HindIII Bluescript KS+ = pCII38. 248 bp SalI/XhoI fragment with 3' end of hsp70 ligated into SalI of Bluescript KS+ = pCIII1. (personal communication) Major Drosophila hsp70 regulator elements are: 2 heat shock consensus elements (-85 to -72 & -62 to -49),a TATA box (-33 to -26) & transcription initiation site (+1) in the 5' segment; the termination triplet and polyA site in the 3' segment. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37642
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified