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Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLR60


Plasmid Type
Cloning Method
ATCC length is 3350 bp. Deposited by: Guillermina Lozano Convenient cloning vector allowing generation of a fusion protein with the DNA-binding domain of GAL4 (aa 3-147). [1] Multiple cloning sites present at both ends of the GAL4 segment permit in-frame fusions with the DNA-binding domain in the middle or at the C-terminus of the fusion product. [1] The order of the major features of this plasmid is: pMB1 ori - lacI (non-functional) - lacZ'/ - T3 promoter - SacI/MCS/SpeI - GAL4 - ClaI/MCS/KpnI - T7 promoter - /lacZ' - f1 ori - ampR. [1] Transcription from the T3 promoter gives the sense strand. [1] pBR322 was modified by deletion of bp 75-2352, destruction (by filling in) of the HindIII site, deletion of a 100 bp AatII/ClaI fragment (nt 4285 and 22 of pBR322), and inserting an XbaI linker into the latter deletion junction. [2] Restriction digests of the clone give the following sizes (kb): SalI--3.35; SacI/KpnI--2.8, 0.5; XhoI--3.1, 0.25. (ATCC staff) Medium is 1227 LB plus ampicillin. GAL4, which is expressed at low levels, encodes a positive regulator of the galactose inducible genes GAL1, GAL2, GAL7, GAL10 and MEL1. GAL4 and the genes it regulates are not linked. Regulation takes place at the transcriptional level. [1] presents data suggesting GAL4 may encode two overlapping proteins. However, the second smaller protein has not yet been identified in vivo. NCBI gi: 171557 Hosts: E.coli, E.coli HB101. Related vectors: pG525. (Information source: VectorDB ( ).)
Catalog Number