Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pNC3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5354
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.1, 1.15; HindIII--5.2; SalI--5.2; PstI--5.2; EcoRI--5.2. (ATCC staff) Expression vector permitting visual detection of recombinants when plated on skim milk indicator plates by the loss or reduction of a halo around a colony. Contains the signal sequence for the neutral protease, so that recombinants in-frame with the gene may be exported. Constructed by an in-frame replacement of part of the pro-peptide coding region with part of the MCS from pUC19. The order of the major features in this plasmid is: nprE (signal peptide coding region - MCS - pro peptide coding region - mature peptide coding region) - ori - kanR. Medium is -1 1065 plus kanamycin (5 ug/ml). Hosts: Bacillus subtilis DB2 trpC2, Bacillus subtilis. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77217
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified