Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pNW33N

Information

Source/Vendor

Bacillus Genetic Stock Center

Plasmid Type

Geobacillus stearothermophilus Expression

Expression Level

Unknown

Cloning Method

Restriction Enzyme

Size

4217

Notes

Note: AmpR fragment is non-functional - Chloramphenicol should be used as the resistance marker.

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

1 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCG 61 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 121 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA 181 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCCCTTC 241 AAACTTCCCA AAGGCGAGCC CTAGTGACAT TAGAAAACCG ACTGTAAAAA GTACAGTCGG 301 CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT 361 CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG 421 TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA 481 ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA 541 AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA 601 TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT 661 CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT 721 ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT 781 TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT 841 GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT 901 CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT 961 CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT 1021 TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATCCCG TTTGTTGAAC TACTCTTTAA 1081 TAAAATAATT TTTCCGTTCC CAATTCCACA TTGCAATAAT AGAAAATCCA TCTTCATCGG 1141 CTTTTTCGTC ATCATCTGTA TGAATCAAAT CGCCTTCTTC TGTGTCATCA AGGTTTAATT 1201 TTTTATGTAT TTCTTTTAAC AAACCACCAT AGGAGATTAA CCTTTTACGG TGTAAACCTT 1261 CCTCCAAATC AGACAAACGT TTCAAATTCT TTTCTTCATC ATCGGTCATA AAATCCGTAT 1321 CCTTTACAGG ATATTTTGCA GTTTCGTCAA TTGCCGATTG TATATCCGAT TTATATTTAT 1381 TTTTCGGTCG AATCATTTGA ACTTTTACAT TTGGATCATA GTCTAATTTC ATTGCCTTTT 1441 TCCAAAATTG AATCCATTGT TTTTGATTCA CGTAGTTTTC TGTATTCTTA AAATAAGTTG 1501 GTTCCACACA TACCAATACA TGCATGTGCT GATTATAAGA ATTATCTTTA TTATTTATTG 1561 TCACTTCCGT TGCACGCATA AAACCAACAA GATTTTTATT AATTTTTTTA TATTGCATCA 1621 TTCGGCGAAA TCCTTGAGCC ATATCTGACA AACTCTTATT TAATTCTTCG CCATCATAAA 1681 CATTTTTAAC TGTTAATGTG AGAAACAACC AACGAACTGT TGGCTTTTGT TTAATAACTT 1741 CAGCAACAAC CTTTTGTGAC TGAATGCCAT GTTTCATTGC TCTCCTCCAG TTGCACATTG 1801 GACAAAGCCT GGATTTACAA AACCACACTC GATACAACTT TCTTTCGCCT GTTTCACGAT 1861 TTTGTTTATA CTCTAATATT TCAGCACAAT CTTTTACTCT TTCAGCCTTT TTAAATTCAA 1921 GAATATGCAG AAGTTCAAAG TAATCAACAT TAGCGATTTT CTTTTCTCTC CATGGTCTCA 1981 CTTTTCCACT TTTTGTCTTG TCCACTAAAA CCCTTGATTT TTCATCTGAA TAAATGCTAC 2041 TATTAGGACA CATAATATTA AAAGAAACCC CCATCTATTT AGTTATTTGT TTGGTCACTT 2101 ATAACTTTAA CAGATGGGGT TTTTCTGTGC AACCAATTTT AAGGGTTTTC AATACTTTAA 2161 AACACATACA TACCAACACT TCAACGCACC TTTCAGCAAC TAAAATAAAA ATGACGTTAT 2221 TTCTATATGT ATCAAGAATA GAAAGAACTC GTTTTTCGCT ACGCTCAAAA CGCAAAAAAA 2281 GCACTCATTC GAGTGCTTTT TCTTATCGCT CCAAATCATG CGATTTTTTC CTCTTTGCTT 2341 TTCTTTGCTC ACGAAGTTCT CGATCACGCT GCAAAACATC TTGAAGCGAA AAAGTATTCT 2401 TCTTTTCTTC CGATCGCTCA TGCTGACGCA CGAAAAGCCC TCTAGGCGCA TAGGAACAAC 2461 TCCTAAATGC ATGTGAGGGG TTTTCTCGTC CATGTGAACA GTCGCATACG CAATATTTTG 2521 TTTCCCATAC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 2581 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 2641 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 2701 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 2761 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 2821 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 2881 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 2941 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 3001 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 3061 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 3121 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 3181 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 3241 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 3301 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 3361 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 3421 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 3481 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 3541 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 3601 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 3661 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCCGATAT 3721 GGGAAACAAA ATATTGCGTA TGCGACTGTT CACATGGACG AGAAAACCCC TCACATGCAT 3781 TTAGGAGTTG TTCCTATGCG CCTAGAGGGC TTTTCGTGCG TCAGCATGAG CGATCGGAAG 3841 AAAAGAAGAA TACTTTTTCG CTTCAAGATG TTTTGCAGCG TGATCGAGAA CTTCGTGAGC 3901 AAAGAAAAGC AAAGAGGAAA AAATCGCATG ATTTGGAGCG ATAAGAAAAA GCACTCGAAT 3961 GAGTGCTTTT TTTGCGTTTT GAGCGTAGCG AAAAACGAGT TCTTTCTATT CTTGATACAT 4021 ATAGAAATAA CGTCATTTTT ATTTTAGTTG CTGAAAGGTG CGTTGAAGTG TTGGTATGTA 4081 TGTGATTCAA TAATTTCTTT TACTCGCTCG TTATAGTCGA TCGGTTCATC ATTCACCAAA 4141 TCATAATTTT CATGTGACCG TTCTTTATCA ATATCGGGAT TCGTTTTACT TTCCCGTTCT 4201 CTCTGATTGT GAAATTG