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PurposeExpresses T7 RNA polymerase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepQE
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Backbone manufacturerQiagen
- Total vector size (bp) 7154
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT7 RNA polymerase
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SpeciesT7 phage
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Insert Size (bp)2685
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE 80L Kan T7WT was a gift from Andrew Ellington (Addgene plasmid # 102790 ; http://n2t.net/addgene:102790 ; RRID:Addgene_102790) -
For your References section:
Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD. Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. 10.1038/nprot.2017.119 PubMed 29120463
Map uploaded by the depositor.
