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pHIV-iRFP720-E2A-Luc
(Plasmid #104587)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104587 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iRFP720-E2A-Luciferase
  • Species
    firefly
  • Insert Size (bp)
    2661
  • Tag / Fusion Protein
    • bi-cistronic (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We constructed this vector from precursor plasmids pHIV-Luciferase (Addgene #21375) and piRFP720-N1 (Addgene #45461)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The vector leads to expression of iRFP720 and luciferase as separate proteins for fluorescence, optoacoustic and bioluminescence imaging.

The full pHIV-iRFP720-E2A-Luc sequence is due to be released by Genbank on 13th Nov. 2017 with accession number MF693179

bioRxiv preprint first posted online Oct. 19, 2017; doi: http://dx.doi.org/10.1101/199836

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-iRFP720-E2A-Luc was a gift from Antonius Plagge (Addgene plasmid # 104587 ; http://n2t.net/addgene:104587 ; RRID:Addgene_104587)
  • For your References section:

    Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes. Comenge J, Sharkey J, Fragueiro O, Wilm B, Brust M, Murray P, Levy R, Plagge A. Elife. 2018 Jun 27;7. pii: 33140. doi: 10.7554/eLife.33140. 10.7554/eLife.33140 PubMed 29949503