pMKO.1 puro GFP shRNA
(Plasmid #10675)

• Depositing Lab: William Hahn
• Publication: Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7.

Backbone

• Vector backbone: pMKO.1
• Backbone size w/o insert (bp): 6700
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP shRNA
• Alt name: GFP
• Species: jellyfish
• Insert Size (bp): 58

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC