pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA
(Plasmid #107050)

• Purpose: All-in-one vectors expressing both SaCas9 and YFP sgRNA
• Depositing Lab: Alex Hewitt
• Publication: Alex Hewitt Lab plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	107050	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SaCas9
• gRNA/shRNA sequence: GTACGTCGCCGTCCAGCTCGAC
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Terms and Licenses:
  • UBMTA

Depositor Comments

YFP sgRNA for SaCas9