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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 11736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBSII KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
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Vector typeCre/Lox ; /Frt
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Selectable markersNeomycin (select with G418) ; Diphtheriatoxin A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepEZ-Frt-lox-DT
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SpeciesDNA derived from yeast, E. Coli, transposon Tn5, Cosynebacterium
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Insert Size (bp)3100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla I (not destroyed)
- 3′ cloning site Don't Know (destroyed during cloning)
- 5′ sequencing primer T7/M13
- 3′ sequencing primer T7/M13 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMark Ansel, CBR Institute for Biomedical Research
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
based on pEasyFlox. Vector for conditional gene targeting
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEZ-Frt-lox-DT was a gift from Klaus Rajewsky (Addgene plasmid # 11736 ; http://n2t.net/addgene:11736 ; RRID:Addgene_11736)