pEZ-Frt-lox-DT
(Plasmid #11736)

• Purpose: Conditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.
• Depositing Lab: Klaus Rajewsky
• Publication: pEZ-Frt-lox-DT (unpublished)

Backbone

• Vector backbone: pBSII KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Cre/Lox ; /Frt
• Selectable markers: Neomycin (select with G418) ; Diphtheriatoxin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pEZ-Frt-lox-DT
• Species: DNA derived from yeast, E. Coli, transposon Tn5, Cosynebacterium
• Insert Size (bp): 3100

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla I (not destroyed)
• 3′ cloning site: Don't Know (destroyed during cloning)
• 5′ sequencing primer: T7/M13
• 3′ sequencing primer: T7/M13

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mark Ansel, CBR Institute for Biomedical Research
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

based on pEasyFlox. Vector for conditional gene targeting