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hSyn1-2xNLS-mTurquoise2
(Plasmid #118025)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118025 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4561
  • Total vector size (bp) 5339
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2xNLS-mTurquoise2
  • Species
    Synthetic
  • Insert Size (bp)
    778
  • Promoter hSyn1
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site CCAAAGAAGAAGCGTAAGGTAgg (unknown if destroyed)
  • 3′ cloning site GCCCCAAGAAaAAGCGgAAGGTgTG (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn1-2xNLS-mTurquoise2 was a gift from Viviana Gradinaru (Addgene plasmid # 118025 ; http://n2t.net/addgene:118025 ; RRID:Addgene_118025)
  • For your References section:

    Systemic AAV vectors for widespread and targeted gene delivery in rodents. Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. 10.1038/s41596-018-0097-3 PubMed 30626963