Systemic AAV vectors for widespread and targeted gene delivery in rodents.
Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V
Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. PubMed Article
Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V
Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104052 | pAAV-CAG-DIO-EYFP | An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter |
| 104053 | pAAV-GFAP-2xNLS-mTurquoise2 | An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter |
| 104054 | pAAV-MBP-2xNLS-tdTomato | An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter |
| 104055 | pAAV-CAG-eYFP | An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter |
| 104056 | pAAV-TRE-eYFP | An AAV genome with tet-inducible expression of the fluorescent protein eYFP |
| 104057 | pAAV-TRE-DIO-eYFP-f | An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence |
| 104058 | CAG-DIO-mRuby2 | AAV expression of Cre-dependent reporter mRuby2 |
| 104059 | CAG-DIO-mTurquoise2 | AAV expression of Cre-dependent reporter mTurquoise2 |
| 104060 | hSyn1-tdTomatof | Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing |
| 104061 | CAG-NLS-GFP | AAV expression of nuclear localized GFP with SV40 NLS |
| 117380 | CAG-eYFP-3x-miR204-5p-TS | An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes |
| 117381 | CAG-eYFP-3x-miR708-5p-TS | An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons |
| 117382 | hSyn1-eYFP | An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter |
| 117383 | TRE-DIO-eYFP | An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP |
| 117384 | CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS | An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively |
| 118025 | hSyn1-2xNLS-mTurquoise2 | An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter |