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Systemic AAV vectors for widespread and targeted gene delivery in rodents.
Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V
Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104052pAAV-CAG-DIO-EYFPAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter
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104053pAAV-GFAP-2xNLS-mTurquoise2An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter
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104054pAAV-MBP-2xNLS-tdTomatoAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter
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104055pAAV-CAG-eYFPAn AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter
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104056pAAV-TRE-eYFPAn AAV genome with tet-inducible expression of the fluorescent protein eYFP
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104057pAAV-TRE-DIO-eYFP-fAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence
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104058CAG-DIO-mRuby2AAV expression of Cre-dependent reporter mRuby2
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104059CAG-DIO-mTurquoise2AAV expression of Cre-dependent reporter mTurquoise2
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104060hSyn1-tdTomatofAlso known as hSyn1-tdTomato-f. AAV expresion of Farnylsated tdtomato for tracing
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104061CAG-NLS-GFPAAV expression of nuclear localized GFP with SV40 NLS
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117380CAG-eYFP-3x-miR204-5p-TSAn AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes
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117381CAG-eYFP-3x-miR708-5p-TSAn AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons
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117382hSyn1-eYFPAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter
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117383TRE-DIO-eYFPAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP
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117384CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TSAn AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
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118025hSyn1-2xNLS-mTurquoise2An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter
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