pISlin41w(s) (lin-41 microRNA wt reporter)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11906||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable at Addgene (plasmid 12178)
- Backbone size w/o insert (bp) 5264
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namelin-41 miRNA target sequence
SpeciesC. elegans (nematode)
Insert Size (bp)103
Entrez Genelin-41 (a.k.a. CELE_C12C8.3)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XbaI/NheI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Used to test effects of lin-41 microRNA (let-7).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pISlin41w(s) (lin-41 microRNA wt reporter) was a gift from David Bartel (Addgene plasmid # 11906 ; http://n2t.net/addgene:11906 ; RRID:Addgene_11906)
For your References section:Prediction of mammalian microRNA targets. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Cell. 2003 Dec 26. 115(7):787-98. 10.1016/S0092-8674(03)01018-3 PubMed 14697198